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ADAR1 p150 isoform is overexpressed in <t>human</t> cancers and correlates with poor prognosis (A) Analysis of TCGA datasets showing ADAR1 p150 mRNA expression levels across cancer types. Data are presented as log2(TPM+1). Statistical significance was determined by unpaired, two-tailed Student’s t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001). (B) Correlation analysis between ADAR1 p150 expression and ESTIMATE immune scores in multiple cancer types. Pearson correlation coefficients and p values are shown. (C) Representative multiplex immunofluorescence images of a representative human colorectal cancer tissue microarray (TMA) core stained for ADAR1 p150 (red), CD8 (cyan), ARG1 (yellow), and PD-L1 (magenta). Scale bar, 50 μm. (D and E) Quantification of spatial associations from the TMA (MC2082d) showing correlations between <t>tumor</t> ADAR1 p150 expression and the abundance of stromal CD8 + T <t>cell,</t> ARG1+ macrophage, and tumor cell PD-L1 expression. Scale bar, 100 μm. Pearson correlation was shown.
Human Tumor Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tumor cells isolation  (Miltenyi Biotec)
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ADAR1 p150 isoform is overexpressed in <t>human</t> cancers and correlates with poor prognosis (A) Analysis of TCGA datasets showing ADAR1 p150 mRNA expression levels across cancer types. Data are presented as log2(TPM+1). Statistical significance was determined by unpaired, two-tailed Student’s t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001). (B) Correlation analysis between ADAR1 p150 expression and ESTIMATE immune scores in multiple cancer types. Pearson correlation coefficients and p values are shown. (C) Representative multiplex immunofluorescence images of a representative human colorectal cancer tissue microarray (TMA) core stained for ADAR1 p150 (red), CD8 (cyan), ARG1 (yellow), and PD-L1 (magenta). Scale bar, 50 μm. (D and E) Quantification of spatial associations from the TMA (MC2082d) showing correlations between <t>tumor</t> ADAR1 p150 expression and the abundance of stromal CD8 + T <t>cell,</t> ARG1+ macrophage, and tumor cell PD-L1 expression. Scale bar, 100 μm. Pearson correlation was shown.
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ADAR1 p150 isoform is overexpressed in <t>human</t> cancers and correlates with poor prognosis (A) Analysis of TCGA datasets showing ADAR1 p150 mRNA expression levels across cancer types. Data are presented as log2(TPM+1). Statistical significance was determined by unpaired, two-tailed Student’s t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001). (B) Correlation analysis between ADAR1 p150 expression and ESTIMATE immune scores in multiple cancer types. Pearson correlation coefficients and p values are shown. (C) Representative multiplex immunofluorescence images of a representative human colorectal cancer tissue microarray (TMA) core stained for ADAR1 p150 (red), CD8 (cyan), ARG1 (yellow), and PD-L1 (magenta). Scale bar, 50 μm. (D and E) Quantification of spatial associations from the TMA (MC2082d) showing correlations between <t>tumor</t> ADAR1 p150 expression and the abundance of stromal CD8 + T <t>cell,</t> ARG1+ macrophage, and tumor cell PD-L1 expression. Scale bar, 100 μm. Pearson correlation was shown.
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ADAR1 p150 isoform is overexpressed in <t>human</t> cancers and correlates with poor prognosis (A) Analysis of TCGA datasets showing ADAR1 p150 mRNA expression levels across cancer types. Data are presented as log2(TPM+1). Statistical significance was determined by unpaired, two-tailed Student’s t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001). (B) Correlation analysis between ADAR1 p150 expression and ESTIMATE immune scores in multiple cancer types. Pearson correlation coefficients and p values are shown. (C) Representative multiplex immunofluorescence images of a representative human colorectal cancer tissue microarray (TMA) core stained for ADAR1 p150 (red), CD8 (cyan), ARG1 (yellow), and PD-L1 (magenta). Scale bar, 50 μm. (D and E) Quantification of spatial associations from the TMA (MC2082d) showing correlations between <t>tumor</t> ADAR1 p150 expression and the abundance of stromal CD8 + T <t>cell,</t> ARG1+ macrophage, and tumor cell PD-L1 expression. Scale bar, 100 μm. Pearson correlation was shown.
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human tumor cell lines jurkat  (ATCC)
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ADAR1 p150 isoform is overexpressed in <t>human</t> cancers and correlates with poor prognosis (A) Analysis of TCGA datasets showing ADAR1 p150 mRNA expression levels across cancer types. Data are presented as log2(TPM+1). Statistical significance was determined by unpaired, two-tailed Student’s t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001). (B) Correlation analysis between ADAR1 p150 expression and ESTIMATE immune scores in multiple cancer types. Pearson correlation coefficients and p values are shown. (C) Representative multiplex immunofluorescence images of a representative human colorectal cancer tissue microarray (TMA) core stained for ADAR1 p150 (red), CD8 (cyan), ARG1 (yellow), and PD-L1 (magenta). Scale bar, 50 μm. (D and E) Quantification of spatial associations from the TMA (MC2082d) showing correlations between <t>tumor</t> ADAR1 p150 expression and the abundance of stromal CD8 + T <t>cell,</t> ARG1+ macrophage, and tumor cell PD-L1 expression. Scale bar, 100 μm. Pearson correlation was shown.
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ADAR1 p150 isoform is overexpressed in <t>human</t> cancers and correlates with poor prognosis (A) Analysis of TCGA datasets showing ADAR1 p150 mRNA expression levels across cancer types. Data are presented as log2(TPM+1). Statistical significance was determined by unpaired, two-tailed Student’s t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001). (B) Correlation analysis between ADAR1 p150 expression and ESTIMATE immune scores in multiple cancer types. Pearson correlation coefficients and p values are shown. (C) Representative multiplex immunofluorescence images of a representative human colorectal cancer tissue microarray (TMA) core stained for ADAR1 p150 (red), CD8 (cyan), ARG1 (yellow), and PD-L1 (magenta). Scale bar, 50 μm. (D and E) Quantification of spatial associations from the TMA (MC2082d) showing correlations between <t>tumor</t> ADAR1 p150 expression and the abundance of stromal CD8 + T <t>cell,</t> ARG1+ macrophage, and tumor cell PD-L1 expression. Scale bar, 100 μm. Pearson correlation was shown.
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ADAR1 p150 isoform is overexpressed in <t>human</t> cancers and correlates with poor prognosis (A) Analysis of TCGA datasets showing ADAR1 p150 mRNA expression levels across cancer types. Data are presented as log2(TPM+1). Statistical significance was determined by unpaired, two-tailed Student’s t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001). (B) Correlation analysis between ADAR1 p150 expression and ESTIMATE immune scores in multiple cancer types. Pearson correlation coefficients and p values are shown. (C) Representative multiplex immunofluorescence images of a representative human colorectal cancer tissue microarray (TMA) core stained for ADAR1 p150 (red), CD8 (cyan), ARG1 (yellow), and PD-L1 (magenta). Scale bar, 50 μm. (D and E) Quantification of spatial associations from the TMA (MC2082d) showing correlations between <t>tumor</t> ADAR1 p150 expression and the abundance of stromal CD8 + T <t>cell,</t> ARG1+ macrophage, and tumor cell PD-L1 expression. Scale bar, 100 μm. Pearson correlation was shown.
Human Tumor Cell Lines Hela, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ADAR1 p150 isoform is overexpressed in human cancers and correlates with poor prognosis (A) Analysis of TCGA datasets showing ADAR1 p150 mRNA expression levels across cancer types. Data are presented as log2(TPM+1). Statistical significance was determined by unpaired, two-tailed Student’s t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001). (B) Correlation analysis between ADAR1 p150 expression and ESTIMATE immune scores in multiple cancer types. Pearson correlation coefficients and p values are shown. (C) Representative multiplex immunofluorescence images of a representative human colorectal cancer tissue microarray (TMA) core stained for ADAR1 p150 (red), CD8 (cyan), ARG1 (yellow), and PD-L1 (magenta). Scale bar, 50 μm. (D and E) Quantification of spatial associations from the TMA (MC2082d) showing correlations between tumor ADAR1 p150 expression and the abundance of stromal CD8 + T cell, ARG1+ macrophage, and tumor cell PD-L1 expression. Scale bar, 100 μm. Pearson correlation was shown.

Journal: iScience

Article Title: Targeting ADAR1 p150 triggers tumor inhibition and antitumor immunity to overcome immunotherapy resistance

doi: 10.1016/j.isci.2026.115978

Figure Lengend Snippet: ADAR1 p150 isoform is overexpressed in human cancers and correlates with poor prognosis (A) Analysis of TCGA datasets showing ADAR1 p150 mRNA expression levels across cancer types. Data are presented as log2(TPM+1). Statistical significance was determined by unpaired, two-tailed Student’s t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001). (B) Correlation analysis between ADAR1 p150 expression and ESTIMATE immune scores in multiple cancer types. Pearson correlation coefficients and p values are shown. (C) Representative multiplex immunofluorescence images of a representative human colorectal cancer tissue microarray (TMA) core stained for ADAR1 p150 (red), CD8 (cyan), ARG1 (yellow), and PD-L1 (magenta). Scale bar, 50 μm. (D and E) Quantification of spatial associations from the TMA (MC2082d) showing correlations between tumor ADAR1 p150 expression and the abundance of stromal CD8 + T cell, ARG1+ macrophage, and tumor cell PD-L1 expression. Scale bar, 100 μm. Pearson correlation was shown.

Article Snippet: Human tumor cell lines, including BJ (CRL-2522) and DLD-1 (CCL-221), were purchased from ATCC.

Techniques: Expressing, Two Tailed Test, Multiplex Assay, Immunofluorescence, Microarray, Staining